Seminaris
  • Medicina Translacional

[TECHtalk] Erich Gnaiger: The NextGen-O2k: extension of high-resolution respirometry by Q-Redox and PhotoBiology Modules

Erich Gnaiger
1. Oroboros Instruments, Innsbruck, Austria
2. Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Austria

 

Oroboros Instruments—as a driving force in mitochondrial physiology—extends the analytical and diagnostic power of high-resolution respirometry by integration of (i) a Q-Redox Module and (ii) a PhotoBiology Module in the NextGen-O2k. The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of the mitochondrial role in human diseases, and mitochondria and chloroplasts ability in regulating algal growth and metabolite production. In just one device researchers obtain a snapshot of the mitochondrial/chloroplast metabolic profile, saving on experimental time, reagents and samples, minimizing errors and ensuring reproducible and accurate results. Substrate-uncoupler-inhibitor titration protocols can be found with the SUITbrowser according to a large diversity of research questions. These SUIT protocols are integral to the DatLab software, which then guides the user through sequences of titration steps and numerous respiratory states in a single assay. In addition, the instrument offers the highest oxygen sensitivity (5 nM detection limit) with an exceptional resolution (wide oxygen concentration range, between 0-1000 μM) and robustness (>10 years lifespan), being superior to any other market option.

 

We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute reproducibility crisis of scientific investigation. In the spirit of Open Science and global networking, we support the COST Action CA15203 MitoEAGLE project to enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the inflation crisis and ultimately the value-impact crisis of present academic publication. This will enhance key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the PhotoBiology Module of the NextGen-O2k, widening our focus from medicine to environment and climate.

                           

 

Supported by Horizon 2020 Framework grant agreement No. 859770 SME instrument phase 2.

 

 

Prof. Erich Gnaiger, is founder and CEO of the SME Oroboros Instruments. He is renowned scientist in the field of mitochondrial physiology. He has scientific and technical expertise in the fields of mitochondrial physiology and pathology, microcalorimetry and biological thermodynamics, contributing fundamentally to the understanding of mitochondrial respiratory control, and to the development and application of high-resolution respirometry in the study of cellular bioenergetics and for the diagnosis of mitochondrial defects. Apart from being CEO of the SME Oroboros Instruments, Prof. Gnaiger also has a position at the D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Austria. Prof. Erich Gnaiger has supervised 12 Ph.D. students to completion and is currently supervising three Ph.D. students in ongoing studies. He is Chair of the COST Action 15203 MitoEAGLE, initiator and chairman of the Mitochondrial Physiology Society (MiP; www.mitophysiology.org), and has organized numerous conferences, workshops, MiPsummer schools and training courses on mitochondrial physiology, bioenergetics and biological thermodynamics.

 

Host: Pablo Garcia-Roves