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[Workshop] Measuring metabolism and bioenergetics in live cells using Seahorse analyzer

Metabolism and bioenergetics are key to understanding cell function and impact on a wide variety of pathways including signaling, proliferation or biosynthesis. Seahorse technology measures mitochondrial respiration and glycolysis in real-time in living cells.  

 

Practical workshop: At the end of the workshop you will be familiar with the main applications and different measurements performed by Seahorse. You will be able to perform experiments with Seahorse in cell cultures and measure oxygen consumption and pH, and interpret the results of mitochondrial function and glycolysis. Please note that registration to the practical workshop is restricted to one person per research group or principal investigator.

 

Please register here: https://forms.office.com/Pages/ResponsePage.aspx?id=6GrqEBX-pkGKsj4DrzJG...

 

Day 1 - 13 November 2019 15:00 - 18:00

 

15:00-18:00 Practical session group 1: Basics of Seahorse - Cartridge preparation and cell culture plating

 

 

 

Day 2 - 14 November 2019 10:00 - 18:00

 

10:00 Presentation of the workshop and Seahorse facility
Sílvia Barceló
Molecular Interactions Unit, Bellvitge Biomedical Research Institute (IDIBELL)
 

 

10:10 Measuring mitochondrial function and glycolysis using the Seahorse XFe96 analyzer
Alfredo Caro
Agilent

 

10:50 Metabolic Flux Analysis - Exploring Oncogenic Mutations in NSCLC Cell Lines
Jaume Morales
Agilent

 

11:30 Coffee break

 

12:00-13:00 Technical seminar for Seahorse users

 

13:00 Lunch
Please note that lunch will not be provided

 

14:30-16:00 Practical session group 1 (lab): Running the experiment

 

16:30-18:00 Practical session group 2 (lab): Cartridge preparation and cell culture plating

 

 

 

Day 3: 15 November 2019 9:30 - 13:30

 

9:30-11:30 Practical session group 2 (lab): Running the experiment

 

11:30 Coffee break

 

12:00-13:30 Practical session groups 1 & 2

Software and data analysis

Questions and discussion

 

 

Instructors: 

Alfredo Caro
Seahorse XF Product Specialist, Agilent Technologies

Jaume Morales
MS Product Specialist, Agilent Technologies
 

 

 

Venue 

 

Seminar: 14 November 2019 10:00 – 12:00

Pau Viladiu Conference Hall

Duran i Reynals Hospital
Avinguda de la Gran Via 199
08907 L’Hospitalet de Llobregat

 

 Practical Session: 14 November 2019 12:00 – 13:00

Lyon room

Duran i Reynals Hospital
Avinguda de la Gran Via 199
08907 L’Hospitalet de Llobregat

 

 

Practical Session: 15 November 2019 12:00 - 13:30

McClintock room

Duran i Reynals Hospital
Avinguda de la Gran Via 199
08907 L’Hospitalet de Llobregat

 

 

Contact:

Silvia Berceló
Molecular Interactions Unit - IDIBELL