Seminario
  • Càncer
  • Medicina Regenerativa
  • Neurociències
  • Medicina Translacional

[Workshop] 3D Cell Culture Models, Genome Editing and Analysis

10:00-11:00 h 3D Models: Developing Better Techniques for Culture and Analysis

 

 

We will go over existing tools, tips, scale up, validated protocols, educational resources as well the challenges and options to improve the analysis of spheroids and organoids. Lastly, we will look at a few examples of the production and use of 3D cell culture models.

 

 

11:00-11:30 h Disease Modelling with Human Pluripotent Stem Cells. Cell Therapy: Pluripotent Stem Cell Workflow

 

 

COFFEE BREAK

 

 

11:50-12:30 h Imaging Modalities for Spheroid and Organoid. Disease-Relevant Assays in iPSC-Derived Cell Types with HCS Platform

 

 

Fluorescence fundamentals, fluorescent reporters, sample preparation, imaging modalities for 3D cultures and principles of high content analysis (HCA) , disease-relevant assays in iPSC-derived cell HCS analysis.

 

 

12:30-13:00 h Genome Editing: CRISPR/Cas9 for Beginners and Advanced Applications

 

 

We will go over the basic steps in how to set up a successful gene editing experiment, as well as some more advanced tools and tips

 

 

13:00-13:45 h QUESTIONS and FREE SAMPLE IMAGING WITH MICROSCOPE EVOS M5000**

 

 

 

Speakers:

 

Christoph Hoefer, M.Sc., Senior Business Development Manager Cell Biology, Thermo Fisher Scientific

 

Carlos Lopez. Ph D., Technical Sales Specialist Microscopy, High Content Analysis & Detection Instruments, Thermo Fisher Scientific

 

 

WE NEED YOU CONFIRM YOUR PARTICIPATION TO arete@idibell.cat

 

** (PLEASE ALSO CONFIRM IF YOU ARE BRINGING YOUR SAMPLE FOR IMAGING with DAPI-GFP-RFP-TxRed)

 

 

Find out more about our 3D portfolio at thermofisher.com/organoid